anti isolectin b4  (Vector Laboratories)

Journal: Acta Neuropathologica Communications

Article Title: Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic stroke

doi: 10.1186/s40478-025-02125-6

Figure Lengend Snippet: Generation of endothelial-specific Rab7a deficient mice. ( A ) Diagrams of the breeding strategy to generate the inducible endothelial Rab7a knockout ( Rab7a iECKO ) mice and the experimental setup to analyze blood-brain barrier (BBB) permeability after t-MCAO. ( B-C” ) Immunofluorescence images for Griffonia (Bandeiraea) Simplicifolia Lectin I (BSL, green), Rab7a protein (red) and DAPI (blue) in the cortex of healthy Rab7a fl/fl (WT, B-B” ) and Rab7a iECKO ( C-C” ) mice. Cortical vessels are delineated by yellow dotted lines ( B’ , C’ ). Boxed areas are magnified in the orthogonal view images on the right ( B” , C” ). Arrowheads indicate endothelial Rab7a, arrows indicate non-endothelial Rab7a. There is no Rab7a protein in brain endothelial cells of mutant mice. Scale bar = 30 μm. (D) Schematic diagram of Ai14 ROSA reporter mice containing a loxP- flanked STOP site before tdTomato to visualize the Cre-mediated recombination efficiency following breeding with the VE-Cadherin-Cre ERT2 mice and 4-OH tamoxifen administration. (E-F’) Immunofluorescence images for Podocalyxin (vessel marker, green), tdTomato (red), and DAPI (blue) in the cortex of Ai14 mice and Ai14 + VE-Cadherin-Cre ERT2 mice show that tdTomato is expressed in all blood vessels following Cre-mediated recombination induced by 4-OH tamoxifen. Normal green arrowhead corresponds to vessels expressing tdTomato, red open arrowhead indicates vessels not expressing tdTomato. Scale bar = 23 μm

Article Snippet: Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine , Vector Labs , Cat #RL-1102-2.

Techniques: Knock-Out, Permeability, Immunofluorescence, Mutagenesis, Marker, Expressing

Journal: Biology

Article Title: High Salt Intake Affects Visceral Adipose Tissue Homeostasis: Beneficial Effects of GLP-1 Agonists

doi: 10.3390/biology14091171

Figure Lengend Snippet: Vascular density measured by a fluorescence assay. ( A ) Representative images of rhodamine-labeled GSL lectin, fluorescein–peanut agglutinin lectin and DAPI–stained sections of representative EAT samples from the Control (A), Control + LGT (B), HSD (C), and HSD + LGT (D) groups. ( B ) Quantification of vascular density and its correlation with the total collagen content (Spearman’s test, n = 10). Data are presented as means ± SDs. One-way ANOVA followed by Bonferroni’s post hoc test. * p < 0.05.

Article Snippet: Then, sections were incubated for 2 h at room temperature with rhodamine-labeled Griffonia simplicifolia lectin (0.12 mg/mL, Cat. #RL-1102, Vector Laboratories, Newark, NJ, USA) to label cell membranes and fluorescein-labeled peanut agglutinin lectin (0.01 mg/mL, Cat. #FL-1071, Vector Laboratories, Newark, NJ, USA) to visualize the interstitial matrix.

Techniques: Fluorescence, Labeling, Staining, Control