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rhodamine labeled griffonia simplicifolia lectin  (Vector Laboratories)


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    Vector Laboratories rhodamine labeled griffonia simplicifolia lectin
    Rhodamine Labeled Griffonia Simplicifolia Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/RL-1102-2/Rhodamine+labeled+Griffonia+(Bandeiraea)+Simplicifolia+Lectin+I+(GSL+I%2C+BSL+I)/pmc13673005-134-9-19
    Average 95 stars, based on 134 article reviews
    rhodamine labeled griffonia simplicifolia lectin - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: High Salt Intake Affects Visceral Adipose Tissue Homeostasis: Beneficial Effects of GLP-1 Agonists
    Article Snippet: Slices were then incubated with a neuraminidase solution (3.3 U/mL, Cat. N#2876, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature. .. Then, sections were incubated for 2 h at room temperature with rhodamine-labeled Griffonia simplicifolia lectin (0.12 mg/mL, Cat. #RL-1102, Vector Laboratories, Newark, NJ, USA) to label cell membranes and fluorescein-labeled peanut agglutinin lectin (0.01 mg/mL, Cat. #FL-1071, Vector Laboratories, Newark, NJ, USA) to visualize the interstitial matrix. .. Nuclei were stained with DAPI (Vectashield ® Cat. #H-1200-10, Vector Laboratories, Newark, NJ, USA).

    Article Title: High Salt Intake Affects Visceral Adipose Tissue Homeostasis: Beneficial Effects of GLP-1 Agonists
    Article Snippet: Slices were then incubated with a neuraminidase solution (3.3 U/mL, Cat. N#2876, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature. .. Then, sections were incubated for 2 h at room temperature with rhodamine-labeled Griffonia simplicifolia lectin (0.12 mg/mL, Cat. #RL-1102, Vector Laboratories, Newark, NJ, USA) to label cell membranes and fluorescein-labeled peanut agglutinin lectin (0.01 mg/mL, Cat. #FL-1071, Vector Laboratories, Newark, NJ, USA) to visualize the interstitial matrix. .. Nuclei were stained with DAPI (Vectashield® Cat. #H-1200-10, Vector Laboratories, Newark, NJ, USA).

    other:

    Article Title: Fibrinogen triggers perivascular fibroblast activation in a mouse model of cortical ischemic stroke
    Article Snippet: Cells were analyzed using the BD FACSDiva 9 (BD Biosciences) and Summit V6.3 (Beckman Coulter Life Sciences) software.

    Staining:

    Article Title: Irradiation of muscle precursor cells impairs the proliferative and angiogenic functions of their extracellular vesicles.
    Article Snippet: .. On day 8, hydrogels were fixed in 4% formaldehyde for 2 h at room temperature, permeabilized using 0.5% Triton-X for 20 min, blocked using 10% goat serum for 2 h, then stained using Rhodamine-labeled Griffonia (Bandeiraea) Simplicifolia Lectin I (GS-1; 1:100; Vector Laboratories, Burlingame, CA). .. The distribution of both vessels and determined using a Leica TCS SP8 Confocal Microscope (Leica, Buffalo Grove, IL) using a rendering of 100 mm thickness/10 mm sections of the entire well coverage area.

    Article Title: Fibro‐Adipogenic Progenitors From Skeletal Muscle Enhance Microvascular Angiogenesis in a 3D Model
    Article Snippet: .. For evaluation of MVF angiogenesis, gels were stained with rhodamine‐labeled Griffonia Simplicifolia Lectin I (GS Lectin I, 1:100, RL‐1102, Vector Laboratories) overnight at 4°C and washed 3 times with 1X PBS. .. A subset of gels containing VF488 + pre‐labeled FAPs was also stained with F‐Actin ( R37112 , Thermo Fisher Scientific) and DAPI ( R37606 , Thermo Fisher Scientific) according to manufacturer protocols to visualize the actin cytoskeleton and nuclei, respectively.



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    Generation of endothelial-specific Rab7a deficient mice. ( A ) Diagrams of the breeding strategy to generate the inducible endothelial Rab7a knockout ( Rab7a iECKO ) mice and the experimental setup to analyze blood-brain barrier (BBB) permeability after t-MCAO. ( B-C” ) Immunofluorescence images for <t>Griffonia</t> <t>(Bandeiraea)</t> <t>Simplicifolia</t> <t>Lectin</t> I (BSL, green), Rab7a protein (red) and DAPI (blue) in the cortex of healthy Rab7a fl/fl (WT, B-B” ) and Rab7a iECKO ( C-C” ) mice. Cortical vessels are delineated by yellow dotted lines ( B’ , C’ ). Boxed areas are magnified in the orthogonal view images on the right ( B” , C” ). Arrowheads indicate endothelial Rab7a, arrows indicate non-endothelial Rab7a. There is no Rab7a protein in brain endothelial cells of mutant mice. Scale bar = 30 μm. (D) Schematic diagram of Ai14 ROSA reporter mice containing a loxP- flanked STOP site before tdTomato to visualize the Cre-mediated recombination efficiency following breeding with the VE-Cadherin-Cre ERT2 mice and 4-OH tamoxifen administration. (E-F’) Immunofluorescence images for Podocalyxin (vessel marker, green), tdTomato (red), and DAPI (blue) in the cortex of Ai14 mice and Ai14 + VE-Cadherin-Cre ERT2 mice show that tdTomato is expressed in all blood vessels following Cre-mediated recombination induced by 4-OH tamoxifen. Normal green arrowhead corresponds to vessels expressing tdTomato, red open arrowhead indicates vessels not expressing tdTomato. Scale bar = 23 μm
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    Generation of endothelial-specific Rab7a deficient mice. ( A ) Diagrams of the breeding strategy to generate the inducible endothelial Rab7a knockout ( Rab7a iECKO ) mice and the experimental setup to analyze blood-brain barrier (BBB) permeability after t-MCAO. ( B-C” ) Immunofluorescence images for <t>Griffonia</t> <t>(Bandeiraea)</t> <t>Simplicifolia</t> <t>Lectin</t> I (BSL, green), Rab7a protein (red) and DAPI (blue) in the cortex of healthy Rab7a fl/fl (WT, B-B” ) and Rab7a iECKO ( C-C” ) mice. Cortical vessels are delineated by yellow dotted lines ( B’ , C’ ). Boxed areas are magnified in the orthogonal view images on the right ( B” , C” ). Arrowheads indicate endothelial Rab7a, arrows indicate non-endothelial Rab7a. There is no Rab7a protein in brain endothelial cells of mutant mice. Scale bar = 30 μm. (D) Schematic diagram of Ai14 ROSA reporter mice containing a loxP- flanked STOP site before tdTomato to visualize the Cre-mediated recombination efficiency following breeding with the VE-Cadherin-Cre ERT2 mice and 4-OH tamoxifen administration. (E-F’) Immunofluorescence images for Podocalyxin (vessel marker, green), tdTomato (red), and DAPI (blue) in the cortex of Ai14 mice and Ai14 + VE-Cadherin-Cre ERT2 mice show that tdTomato is expressed in all blood vessels following Cre-mediated recombination induced by 4-OH tamoxifen. Normal green arrowhead corresponds to vessels expressing tdTomato, red open arrowhead indicates vessels not expressing tdTomato. Scale bar = 23 μm
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    Image Search Results


    Generation of endothelial-specific Rab7a deficient mice. ( A ) Diagrams of the breeding strategy to generate the inducible endothelial Rab7a knockout ( Rab7a iECKO ) mice and the experimental setup to analyze blood-brain barrier (BBB) permeability after t-MCAO. ( B-C” ) Immunofluorescence images for Griffonia (Bandeiraea) Simplicifolia Lectin I (BSL, green), Rab7a protein (red) and DAPI (blue) in the cortex of healthy Rab7a fl/fl (WT, B-B” ) and Rab7a iECKO ( C-C” ) mice. Cortical vessels are delineated by yellow dotted lines ( B’ , C’ ). Boxed areas are magnified in the orthogonal view images on the right ( B” , C” ). Arrowheads indicate endothelial Rab7a, arrows indicate non-endothelial Rab7a. There is no Rab7a protein in brain endothelial cells of mutant mice. Scale bar = 30 μm. (D) Schematic diagram of Ai14 ROSA reporter mice containing a loxP- flanked STOP site before tdTomato to visualize the Cre-mediated recombination efficiency following breeding with the VE-Cadherin-Cre ERT2 mice and 4-OH tamoxifen administration. (E-F’) Immunofluorescence images for Podocalyxin (vessel marker, green), tdTomato (red), and DAPI (blue) in the cortex of Ai14 mice and Ai14 + VE-Cadherin-Cre ERT2 mice show that tdTomato is expressed in all blood vessels following Cre-mediated recombination induced by 4-OH tamoxifen. Normal green arrowhead corresponds to vessels expressing tdTomato, red open arrowhead indicates vessels not expressing tdTomato. Scale bar = 23 μm

    Journal: Acta Neuropathologica Communications

    Article Title: Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic stroke

    doi: 10.1186/s40478-025-02125-6

    Figure Lengend Snippet: Generation of endothelial-specific Rab7a deficient mice. ( A ) Diagrams of the breeding strategy to generate the inducible endothelial Rab7a knockout ( Rab7a iECKO ) mice and the experimental setup to analyze blood-brain barrier (BBB) permeability after t-MCAO. ( B-C” ) Immunofluorescence images for Griffonia (Bandeiraea) Simplicifolia Lectin I (BSL, green), Rab7a protein (red) and DAPI (blue) in the cortex of healthy Rab7a fl/fl (WT, B-B” ) and Rab7a iECKO ( C-C” ) mice. Cortical vessels are delineated by yellow dotted lines ( B’ , C’ ). Boxed areas are magnified in the orthogonal view images on the right ( B” , C” ). Arrowheads indicate endothelial Rab7a, arrows indicate non-endothelial Rab7a. There is no Rab7a protein in brain endothelial cells of mutant mice. Scale bar = 30 μm. (D) Schematic diagram of Ai14 ROSA reporter mice containing a loxP- flanked STOP site before tdTomato to visualize the Cre-mediated recombination efficiency following breeding with the VE-Cadherin-Cre ERT2 mice and 4-OH tamoxifen administration. (E-F’) Immunofluorescence images for Podocalyxin (vessel marker, green), tdTomato (red), and DAPI (blue) in the cortex of Ai14 mice and Ai14 + VE-Cadherin-Cre ERT2 mice show that tdTomato is expressed in all blood vessels following Cre-mediated recombination induced by 4-OH tamoxifen. Normal green arrowhead corresponds to vessels expressing tdTomato, red open arrowhead indicates vessels not expressing tdTomato. Scale bar = 23 μm

    Article Snippet: Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine , Vector Labs , Cat #RL-1102-2.

    Techniques: Knock-Out, Permeability, Immunofluorescence, Mutagenesis, Marker, Expressing